Picto_Plasma FAQ

 

Questions on THERAFLEX MB-PLASMA process

 

What type of plasma should be used with THERAFLEX MB-Plasma?

Both whole blood and Aphaeresis plasma.

In case of distribution of plasmaphaeresis, which is the lowest volume of plasma that can be inactivated with the THERAFLEX kit? Macopharma recommends a volume range of 235-330 ml before plasma filtration. It is preferable to split and filter at the same time the apheresis plasma (455-645 ml), using the BRV8000A kit (triple PLAS4 distribution kit), then to connect each of the units to a single MB kit (ZDV configuration) without PLAS4. This allows the loss of plasma due to filtration to be as low as possible, and to distribute 3 MB-treated units from aphaeresis plasma.

 

 

Is there a delay to respect for illumination after MB pill dissolution?

The illumination should be started 90 min max after MB pill addition.

 

 

During the delay between MB pill dissolution and illumination should we keep plasma bags exposed to daylight?

No, you should not expose to light during this period. We recommend you to do the illumination directly after MB dissolution or if you should wait we recommend you to keep plasma bag away from light (to cover them for example).

 

 

What is the duration of illumination time?

With the Macotronic V4 (under the normal conditions) the illumination time is between 20 and 25 minutes. With the Macotronic B2 (under the normal conditions) the illumination time is between 15 and 20 minutes.

 

 

When should we start Blueflex filtration?

Start BLUELFEX filtration max. 60 min after successful illumination.

 

 

After Blueflex filtration should we keep inactivated plasma bag exposed to daylight?

No, do not expose to light during this period.

 

 

What is the maximum storage time for the treated plasma?

Treated plasma can be stored as long as untreated plasma.

 

 

Should we keep plasma bags (mixed with Methylene Blue) protected from intensive light before and after treatment?

Yes, do not expose to light during these periods.

 

 

Why should we not keep plasma bags exposed to intensive light?

Because we might have a loss in Fibrinogen content. This influence was investigated and it was shown that illumination with strong light doses (sunlight; 25.000 Lux) for 60 min before and/or after treatment could reduce functional fibrinogen by 10-14 %. Moderate light doses (10.000 Lux) have only a minor detrimental effect.

 

 

Should we keep inactivated plasma samples (for quality control) protected from intensive light after treatment?

Yes, do not expose to light during this period.

 

 

Why should we not keep inactivated plasma samples exposed to intensive light?

Because we might have a loss in Fibrinogen content (13% to 25% loss if exposed to light doses for 60 min). In all cases the validation or quality control data and acceptance criteria of the respective blood bank under their routine production conditions are pivotal.

 

 

How do we protect plasma bags and plasma samples from intensive light during the MB procedure?

To avoid higher Fibrinogen loss during the MB procedure, you should respect the following instructions:

  • Put the bags into a box,
  • Place the bags into a drawer or,
  • Store the bags in a dark room before and after illumination.